Primer

Part:BBa_K4615006

Designed by: Xinyi (Marry) Xuan   Group: iGEM23_Toronto   (2023-10-12)


Reverse primer to insert 20 bp of sgRNA of frdA into pTargetF

This part will amplify the backbone of pTargetF along with targeted frdA sgRNA sequence to generalize linear PCR fragments, products will be transformed into E.Coli later and self-circulaized in T-B18.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None