![](https://parts.igem.org/images/partbypart/icon_primer.png)
Primer
Part:BBa_K4615006
Designed by: Xinyi (Marry) Xuan Group: iGEM23_Toronto (2023-10-12)
Reverse primer to insert 20 bp of sgRNA of frdA into pTargetF
This part will amplify the backbone of pTargetF along with targeted frdA sgRNA sequence to generalize linear PCR fragments, products will be transformed into E.Coli later and self-circulaized in T-B18.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |